VASP ELISA Kit

Produktnummer ABIN5659905

Produktdetails VASP ELISA Kit

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Vasodilator Stimulated Phosphoprotein (VASP). No significant cross-reactivity or interference between Vasodilator Stimulated Phosphoprotein (VASP) and analogues was observed.

Sensitivität: 0.056 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vasodilator Stimulated Phosphoprotein (VASP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vasodilator Stimulated Phosphoprotein (VASP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vasodilator Stimulated Phosphoprotein (VASP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vasodilator Stimulated Phosphoprotein (VASP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vasodilator Stimulated Phosphoprotein (VASP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vasodilator Stimulated Phosphoprotein (VASP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu VASP

Target: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Andere Bezeichnung: Vasodilator Stimulated Phosphoprotein (VASP Produkte)

Synonyme: vasp ELISA Kit, MGC80889 ELISA Kit, wu:fb23b04 ELISA Kit, wu:fk84g05 ELISA Kit, zgc:110347 ELISA Kit, si:dkey-113g17.1 ELISA Kit, si:ch211-202c21.1 ELISA Kit, DDBDRAFT_0188463 ELISA Kit, DDBDRAFT_0229340 ELISA Kit, DDB_0188463 ELISA Kit, DDB_0229340 ELISA Kit, VASP ELISA Kit, vasodilator stimulated phosphoprotein S homeolog ELISA Kit, vasodilator stimulated phosphoprotein b ELISA Kit, protein enabled ELISA Kit, vasodilator-stimulated phosphoprotein ELISA Kit, vasodilator stimulated phosphoprotein a ELISA Kit, vasodilator stimulated phosphoprotein ELISA Kit, vasp.S ELISA Kit, vaspb ELISA Kit, LOC5573842 ELISA Kit, CpipJ_CPIJ004706 ELISA Kit, CpipJ_CPIJ004707 ELISA Kit, vasP ELISA Kit, vasp ELISA Kit, VASP ELISA Kit, vaspa ELISA Kit, Vasp ELISA Kit

Hintergrund:

Gene Name: Vasodilator Stimulated Phosphoprotein

Pathways: T-Zell Rezeptor Signalweg, Regulation of Actin Filament Polymerization, Tube Formation