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WT1 CLIA Kit

WT1 Reaktivität: Human Chemiluminescent Sandwich ELISA 156.25 pg/mL - 10000 pg/mL Tissue Homogenate
Produktnummer ABIN5659991
  • Target Alle WT1 CLIA Kits anzeigen
    WT1 (Wilms Tumor 1 (WT1))
    Reaktivität
    • 3
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Chemiluminescent
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    156.25 pg/mL - 10000 pg/mL
    Untere Nachweisgrenze
    156.25 pg/mL
    Applikation
    ELISA
    Proben
    Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Wilms Tumor Protein (WT1). No significant cross-reactivity or interference between Wilms Tumor Protein (WT1) and analogues was observed.
    Sensitivität
    6.21 pg/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    2 - 3 h
    Plattentyp
    Pre-coated
    Protokoll
    The microplate provided in this kit has been pre-coated with an antibody specific to Wilms Tumor Protein (WT1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Wilms Tumor Protein (WT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Wilms Tumor Protein (WT1) level in the sample or standard.,
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wilms Tumor Protein (WT1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wilms Tumor Protein (WT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle WT1 CLIA Kits anzeigen
    WT1 (Wilms Tumor 1 (WT1))
    Andere Bezeichnung
    Wilms Tumor Protein (WT1 Produkte)
    Synonyme
    AWT1 CLIA Kit, EWS-WT1 CLIA Kit, GUD CLIA Kit, NPHS4 CLIA Kit, WAGR CLIA Kit, WIT-2 CLIA Kit, WT33 CLIA Kit, CWT1 CLIA Kit, WT1 CLIA Kit, XWT1a CLIA Kit, wt-1 CLIA Kit, wt1 CLIA Kit, xWT1 CLIA Kit, WT-1 CLIA Kit, gud CLIA Kit, wagr CLIA Kit, wt33 CLIA Kit, xwt1 CLIA Kit, wit-2 CLIA Kit, si:ch211-160j6.3 CLIA Kit, zgc:152697 CLIA Kit, D630046I19Rik CLIA Kit, Wt-1 CLIA Kit, XWT1b CLIA Kit, XeWT1 CLIA Kit, Wilms tumor 1 CLIA Kit, Wilms tumor 1 S homeolog CLIA Kit, wilms tumor 1b CLIA Kit, Wilms tumor protein CLIA Kit, Wilms tumor 1 homolog CLIA Kit, Wilms tumor 1 L homeolog CLIA Kit, WT1 CLIA Kit, wt1.S CLIA Kit, wt1 CLIA Kit, wt1b CLIA Kit, Wt1 CLIA Kit, wt1.L CLIA Kit
    Hintergrund

    Gene Name: Wilms Tumor Protein

    Gene Aliases: WAGR, GUD, WIT-2, WT33

    Gen-ID
    7490
    UniProt
    P19544
    Pathways
    Tube Formation
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