WT1 ELISA Kit

Produktnummer ABIN5659992

Produktdetails WT1 ELISA Kit

Target: WT1 (Wilms Tumor 1 (WT1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Wilms Tumor Protein (WT1). No significant cross-reactivity or interference between Wilms Tumor Protein (WT1) and analogues was observed.

Sensitivität: 0.063 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wilms Tumor Protein (WT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wilms Tumor Protein (WT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wilms Tumor Protein (WT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wilms Tumor Protein (WT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wilms Tumor Protein (WT1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wilms Tumor Protein (WT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Andere Bezeichnung: Wilms Tumor Protein (WT1 Produkte)

Synonyme: AWT1 ELISA Kit, EWS-WT1 ELISA Kit, GUD ELISA Kit, NPHS4 ELISA Kit, WAGR ELISA Kit, WIT-2 ELISA Kit, WT33 ELISA Kit, CWT1 ELISA Kit, WT1 ELISA Kit, XWT1a ELISA Kit, wt-1 ELISA Kit, wt1 ELISA Kit, xWT1 ELISA Kit, WT-1 ELISA Kit, gud ELISA Kit, wagr ELISA Kit, wt33 ELISA Kit, xwt1 ELISA Kit, wit-2 ELISA Kit, si:ch211-160j6.3 ELISA Kit, zgc:152697 ELISA Kit, D630046I19Rik ELISA Kit, Wt-1 ELISA Kit, XWT1b ELISA Kit, XeWT1 ELISA Kit, Wilms tumor 1 ELISA Kit, Wilms tumor 1 S homeolog ELISA Kit, wilms tumor 1b ELISA Kit, Wilms tumor protein ELISA Kit, Wilms tumor 1 homolog ELISA Kit, Wilms tumor 1 L homeolog ELISA Kit, WT1 ELISA Kit, wt1.S ELISA Kit, wt1 ELISA Kit, wt1b ELISA Kit, Wt1 ELISA Kit, wt1.L ELISA Kit

Hintergrund:

Gene Name: Wilms Tumor Protein

Gene Aliases: WAGR, GUD, WIT-2, WT33

Gen-ID: 7490

UniProt: P19544

Pathways: Tube Formation