XDH CLIA Kit

Produktnummer ABIN5660059

Produktdetails XDH CLIA Kit

Target: XDH (Xanthine Dehydrogenase (XDH))

Reaktivität: Rind (Kuh)

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.62 pg/mL - 1000 pg/mL

Untere Nachweisgrenze: 15.62 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Xanthine Dehydrogenase (XDH). No significant cross-reactivity or interference between Xanthine Dehydrogenase (XDH) and analogues was observed.

Sensitivität: 0.58 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Xanthine Dehydrogenase (XDH). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Xanthine Dehydrogenase (XDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Xanthine Dehydrogenase (XDH) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Xanthine Dehydrogenase (XDH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Xanthine Dehydrogenase (XDH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: XDH (Xanthine Dehydrogenase (XDH))

Andere Bezeichnung: Xanthine Dehydrogenase (XDH Produkte)

Synonyme: XO CLIA Kit, XOR CLIA Kit, Xor CLIA Kit, Xox-1 CLIA Kit, Xox1 CLIA Kit, XDH CLIA Kit, im:7146167 CLIA Kit, DDBDRAFT_0219626 CLIA Kit, DDBDRAFT_0230176 CLIA Kit, DDB_0219626 CLIA Kit, DDB_0230176 CLIA Kit, xanthine dehydrogenase CLIA Kit, xanthine dehydrogenase/oxidase CLIA Kit, Xanthine dehydrogenase CLIA Kit, XDH CLIA Kit, Xdh CLIA Kit, xdh CLIA Kit, LOC576712 CLIA Kit, Trebr_1754 CLIA Kit

Hintergrund:

Gene Name: Xanthine Dehydrogenase

Gene Aliases: XO, XOR, XOD, Xanthine Oxidase, NAD+-Xanthine Dehydrogenase, Xanthine-NAD+ Oxidoreductase, Xanthine/NAD+ Oxidoreductase, Xanthine Oxidoreductase

Gen-ID: 280960

UniProt: P80457

Pathways: Positive Regulation of Endopeptidase Activity