XDH ELISA Kit

Produktnummer ABIN5660060

Produktdetails XDH ELISA Kit

Target: XDH (Xanthine Dehydrogenase (XDH))

Reaktivität: Rind (Kuh)

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.781 ng/mL - 50 ng/mL

Untere Nachweisgrenze: 0.781 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Xanthine Dehydrogenase (XDH). No significant cross-reactivity or interference between Xanthine Dehydrogenase (XDH) and analogues was observed.

Sensitivität: 0.27 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Xanthine Dehydrogenase (XDH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Xanthine Dehydrogenase (XDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Xanthine Dehydrogenase (XDH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Xanthine Dehydrogenase (XDH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Xanthine Dehydrogenase (XDH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Xanthine Dehydrogenase (XDH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu XDH

Target: XDH (Xanthine Dehydrogenase (XDH))

Andere Bezeichnung: Xanthine Dehydrogenase (XDH Produkte)

Synonyme: XO ELISA Kit, XOR ELISA Kit, Xor ELISA Kit, Xox-1 ELISA Kit, Xox1 ELISA Kit, XDH ELISA Kit, im:7146167 ELISA Kit, DDBDRAFT_0219626 ELISA Kit, DDBDRAFT_0230176 ELISA Kit, DDB_0219626 ELISA Kit, DDB_0230176 ELISA Kit, xanthine dehydrogenase ELISA Kit, xanthine dehydrogenase/oxidase ELISA Kit, Xanthine dehydrogenase ELISA Kit, XDH ELISA Kit, Xdh ELISA Kit, xdh ELISA Kit, LOC576712 ELISA Kit, Trebr_1754 ELISA Kit

Hintergrund:

Gene Name: Xanthine Dehydrogenase

Gene Aliases: XO, XOR, XOD, Xanthine Oxidase, NAD+-Xanthine Dehydrogenase, Xanthine-NAD+ Oxidoreductase, Xanthine/NAD+ Oxidoreductase, Xanthine Oxidoreductase

Gen-ID: 280960

UniProt: P80457

Pathways: Positive Regulation of Endopeptidase Activity