YBX1 ELISA Kit

Produktnummer ABIN5660073

Produktdetails YBX1 ELISA Kit

Target: YBX1 (Y Box Binding Protein 1 (YBX1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Y-Box Binding Protein 1 (YBX1). No significant cross-reactivity or interference between Y-Box Binding Protein 1 (YBX1) and analogues was observed.

Sensitivität: 0.063 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Y-Box Binding Protein 1 (YBX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Y-Box Binding Protein 1 (YBX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Y-Box Binding Protein 1 (YBX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Y-Box Binding Protein 1 (YBX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Y-Box Binding Protein 1 (YBX1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Y-Box Binding Protein 1 (YBX1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu YBX1

Target: YBX1 (Y Box Binding Protein 1 (YBX1))

Andere Bezeichnung: Y-Box Binding Protein 1 (YBX1 Produkte)

Synonyme: BP-8 ELISA Kit, CSDA2 ELISA Kit, CSDB ELISA Kit, DBPB ELISA Kit, MDR-NF1 ELISA Kit, NSEP-1 ELISA Kit, NSEP1 ELISA Kit, YB-1 ELISA Kit, YB1 ELISA Kit, Byb1 ELISA Kit, Cbfa ELISA Kit, Dbpb ELISA Kit, Ef1a ELISA Kit, Msy1 ELISA Kit, Nsep1 ELISA Kit, Yb1 ELISA Kit, bp-8 ELISA Kit, csda2 ELISA Kit, csdb ELISA Kit, dbpb ELISA Kit, frgy1 ELISA Kit, mdr-nf1 ELISA Kit, nsep-1 ELISA Kit, nsep1 ELISA Kit, yb1 ELISA Kit, 1700102N10Rik ELISA Kit, C79409 ELISA Kit, EF1A ELISA Kit, MSY1 ELISA Kit, dbpB ELISA Kit, mYB-1a ELISA Kit, nuclease sensitive element binding protein 1 ELISA Kit, Y-box binding protein 1 ELISA Kit, Y box binding protein 1 ELISA Kit, Y-box binding protein 1 S homeolog ELISA Kit, nuclease-sensitive element-binding protein 1 ELISA Kit, Y box protein 1 ELISA Kit, nsep1 ELISA Kit, YBX1 ELISA Kit, Ybx1 ELISA Kit, ybx1.S ELISA Kit, LOC479011 ELISA Kit

Hintergrund:

Gene Name: Y-Box Binding Protein 1

Gene Aliases: BP8, CSDA2, CSDB, DBPB, MDR-NF1, NSEP1, YB1, Nuclease Sensitive Element Binding Protein 1, CCAAT-binding transcription factor I subunit A, DNA-binding protein B

Pathways: Regulation of Muscle Cell Differentiation