LDLRAP1 ELISA Kit

Produktnummer ABIN5674658

Produktdetails LDLRAP1 ELISA Kit

Target: LDLRAP1 (Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0056 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu LDLRAP1

Target: LDLRAP1 (Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1))

Abstract: LDLRAP1 Produkte

Synonyme: ARH ELISA Kit, ARH1 ELISA Kit, ARH2 ELISA Kit, FHCB1 ELISA Kit, FHCB2 ELISA Kit, AA691260 ELISA Kit, Arh ELISA Kit, Arh1 ELISA Kit, RGD1563417 ELISA Kit, arh ELISA Kit, arh1 ELISA Kit, arh2 ELISA Kit, fhcb1 ELISA Kit, fhcb2 ELISA Kit, xptb ELISA Kit, LDLRAP1 ELISA Kit, ldlrap1 ELISA Kit, sb:cb50 ELISA Kit, zgc:56121 ELISA Kit, zgc:158745 ELISA Kit, low density lipoprotein receptor adaptor protein 1 ELISA Kit, low density lipoprotein receptor adaptor protein 1 L homeolog ELISA Kit, low density lipoprotein receptor adaptor protein 1 S homeolog ELISA Kit, low density lipoprotein receptor adaptor protein 1a ELISA Kit, low density lipoprotein receptor adaptor protein 1b ELISA Kit, LDLRAP1 ELISA Kit, Ldlrap1 ELISA Kit, ldlrap1 ELISA Kit, ldlrap1.L ELISA Kit, ldlrap1.S ELISA Kit, ldlrap1a ELISA Kit, ldlrap1b ELISA Kit

Hintergrund: Alternative name: ARH1, ARH, ARH2, FHCB1, FHCB2, Autosomal recessive hypercholesterolemia protein

Gen-ID: 26119

UniProt: Q5SW96

Pathways: Lipid Metabolism