ATP1A1 ELISA Kit
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- Target Alle ATP1A1 ELISA Kits anzeigen
- ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 78.125 pg/mL - 5000 pg/mL
- Untere Nachweisgrenze
- 78.125 pg/mL
- Applikation
- ELISA
- Proben
- Tissue Homogenate
- Analytische Methode
- Quantitative
- Sensitivität
- 26.6 pg/mL
- Bestandteile
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - Benötigtes Material
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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Sodium Potassium ATPase, alpha1 (ATP1A1) ELISA Kit
ATP1A1 Reaktivität: Human, Ratte, Maus, Schwein Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Sodium Potassium ATPase, alpha1 (ATP1A1) ELISA KitATP1A1 Reaktivität: Kaninchen Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Sodium Potassium ATPase, alpha1 (ATP1A1) ELISA KitATP1A1 Reaktivität: Ratte Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Testdauer
- 1 - 4.5 h
- Plattentyp
- Pre-coated
- Protokoll
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- 4 °C
- Haltbarkeit
- 12 months
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- Target Alle ATP1A1 ELISA Kits anzeigen
- ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))
- Andere Bezeichnung
- ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) (ATP1A1 Produkte)
- Hintergrund
- Alternative name: Sodium pump subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium/potassium-transporting ATPase subunit alpha-1
- Gen-ID
- 476
- UniProt
- P05023
- Pathways
- Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Proton Transport, Ribonucleoside Biosynthetic Process
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