NDRG2 ELISA Kit

Produktnummer ABIN5674701

Produktdetails NDRG2 ELISA Kit

Target: NDRG2 (NDRG Family Member 2 (NDRG2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0115 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu NDRG2

Target: NDRG2 (NDRG Family Member 2 (NDRG2))

Andere Bezeichnung: N-myc Downstream Regulated Gene 2 (NDRG2) (NDRG2 Produkte)

Synonyme: NDRG2 ELISA Kit, AI182517 ELISA Kit, AU040374 ELISA Kit, Ndr2 ELISA Kit, SYLD ELISA Kit, im:6909381 ELISA Kit, si:dkey-88n24.1 ELISA Kit, zgc:101847 ELISA Kit, NDRG family member 2 ELISA Kit, N-myc downstream regulated gene 2 ELISA Kit, NDRG family member 2 S homeolog ELISA Kit, ndrg2 ELISA Kit, NDRG2 ELISA Kit, Ndrg2 ELISA Kit, ndrg2.S ELISA Kit

Hintergrund: Alternative name: SYLD, N-myc downstream-regulated gene 2 protein

Gen-ID: 57447

UniProt: Q9UN36