NCDN ELISA Kit

Produktnummer ABIN5674713

Produktdetails NCDN ELISA Kit

Target: NCDN (Neurochondrin (NCDN))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0058 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu NCDN

Target: NCDN (Neurochondrin (NCDN))

Abstract: NCDN Produkte

Synonyme: CG2330 ELISA Kit, Dmel\\CG2330 ELISA Kit, NCDN ELISA Kit, GB14786 ELISA Kit, AU042419 ELISA Kit, MMS10-AE ELISA Kit, Ms10ae ELISA Kit, mKIAA0607 ELISA Kit, norbin ELISA Kit, Norbin ELISA Kit, CG2330 gene product from transcript CG2330-RA ELISA Kit, neurochondrin ELISA Kit, N-acylneuraminate-9-phosphatase ELISA Kit, neurochondrin S homeolog ELISA Kit, Neurochondrin ELISA Kit, NCDN ELISA Kit, LOC552430 ELISA Kit, ncdn ELISA Kit, Ncdn ELISA Kit, ncdn.S ELISA Kit

Hintergrund: Alternative name: Norbin

Gen-ID: 26562

UniProt: Q9Z0E0