SOCS1 ELISA Kit

Produktnummer ABIN5674725

Produktdetails SOCS1 ELISA Kit

Target: SOCS1 (Suppressor of Cytokine Signaling 1 (SOCS1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0.14 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu SOCS1

Target: SOCS1 (Suppressor of Cytokine Signaling 1 (SOCS1))

Andere Bezeichnung: Suppressors Of Cytokine Signaling 1 (SOCS1) (SOCS1 Produkte)

Synonyme: socs1 ELISA Kit, zgc:91868 ELISA Kit, jab ELISA Kit, cis1 ELISA Kit, ssi1 ELISA Kit, tip3 ELISA Kit, cish1 ELISA Kit, ssi-1 ELISA Kit, socs-1 ELISA Kit, SOCS1 ELISA Kit, LOC100228306 ELISA Kit, socs1b ELISA Kit, CIS1 ELISA Kit, CISH1 ELISA Kit, JAB ELISA Kit, SOCS-1 ELISA Kit, SSI-1 ELISA Kit, SSI1 ELISA Kit, TIP3 ELISA Kit, Cish1 ELISA Kit, Cish7 ELISA Kit, Socs-1 ELISA Kit, suppressor of cytokine signaling 1a ELISA Kit, suppressor of cytokine signaling 1 ELISA Kit, suppressor of cytokine signaling 1 L homeolog ELISA Kit, socs1a ELISA Kit, socs1 ELISA Kit, SOCS1 ELISA Kit, socs1.L ELISA Kit, Socs1 ELISA Kit

Hintergrund: Alternative name: CIS1, CISH1, JAB, SOCS-1, SSI-1, SSI1, TIP3, JAK-binding protein, STAT-induced STAT inhibitor 1, Tec-interacting protein 3

Gen-ID: 12703

UniProt: O35716

Pathways: JAK-STAT Signalweg, Interferon-gamma Pathway, TLR Signalweg, Response to Growth Hormone Stimulus