WBP2NL ELISA Kit

Produktnummer ABIN5674737

Produktdetails WBP2NL ELISA Kit

Target: WBP2NL (WBP2 N-terminal Like (WBP2NL))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25 pg/mL - 2000 pg/mL

Untere Nachweisgrenze: 31.25 pg/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 12.6 pg/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu WBP2NL

Target: WBP2NL (WBP2 N-terminal Like (WBP2NL))

Andere Bezeichnung: Postacrosomal Sheath WW Domain Binding Protein (PAWP) (WBP2NL Produkte)

Synonyme: pawp ELISA Kit, PAWP ELISA Kit, 4930521I23Rik ELISA Kit, WBP2 N-terminal like ELISA Kit, WBP2 N-terminal like L homeolog ELISA Kit, Wbp2nl ELISA Kit, wbp2nl.L ELISA Kit, wbp2nl ELISA Kit, WBP2NL ELISA Kit

Hintergrund: Alternative name: WBP2NL, WBP2 N-Terminal Like, WW domain-binding protein 2-like

Gen-ID: 74716

UniProt: Q9D529