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Peripherin ELISA Kit

PRPH Reaktivität: Human Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5674744
  • Target Alle Peripherin (PRPH) ELISA Kits anzeigen
    Peripherin (PRPH)
    Reaktivität
    • 3
    • 2
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    78.125 pg/mL - 5000 pg/mL
    Untere Nachweisgrenze
    78.125 pg/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Sensitivität
    32.7 pg/mL
    Bestandteile
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Benötigtes Material
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Testdauer
    1 - 4.5 h
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Haltbarkeit
    12 months
  • Target Alle Peripherin (PRPH) ELISA Kits anzeigen
    Peripherin (PRPH)
    Abstract
    PRPH Produkte
    Synonyme
    NEF4 ELISA Kit, PRPH1 ELISA Kit, Prph1 ELISA Kit, Perf ELISA Kit, nef4 ELISA Kit, prph1 ELISA Kit, MGC69454 ELISA Kit, if3 ELISA Kit, plasticin ELISA Kit, zgc:111926 ELISA Kit, peripherin ELISA Kit, peripherin L homeolog ELISA Kit, PRPH ELISA Kit, Prph ELISA Kit, prph ELISA Kit, prph.L ELISA Kit
    Hintergrund
    Alternative name: NEF4, Neurofilament 4
    Gen-ID
    5630
    UniProt
    P41219
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