APEX1 ELISA Kit

Produktnummer ABIN5674748

Produktdetails APEX1 ELISA Kit

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0143 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Andere Bezeichnung: APEX Nuclease 1 (APEX1) (APEX1 Produkte)

Synonyme: apex1 ELISA Kit, MGC76280 ELISA Kit, MGC79645 ELISA Kit, MGC78928 ELISA Kit, APE ELISA Kit, APE1 ELISA Kit, APEN ELISA Kit, APEX ELISA Kit, APX ELISA Kit, HAP1 ELISA Kit, REF1 ELISA Kit, Apex ELISA Kit, REF-1 ELISA Kit, BAP1 ELISA Kit, apex1a ELISA Kit, hm:zehn1532 ELISA Kit, wu:fc13d11 ELISA Kit, zgc:66204 ELISA Kit, Ref-1 ELISA Kit, apurinic/apyrimidinic endodeoxyribonuclease 1 ELISA Kit, APEX nuclease (multifunctional DNA repair enzyme) 1 ELISA Kit, APEX nuclease (multifunctional DNA repair enzyme) 1 L homeolog ELISA Kit, DNA-(apurinic or apyrimidinic site) lyase ELISA Kit, apurinic/apyrimidinic endonuclease 1 ELISA Kit, APEX1 ELISA Kit, apex1 ELISA Kit, apex1.L ELISA Kit, CNG03640 ELISA Kit, CNH01280 ELISA Kit, nth ELISA Kit, Spirs_4119 ELISA Kit, Palpr_0103 ELISA Kit, Apex1 ELISA Kit

Hintergrund: Alternative name: APE, APE1, APEN, APEX, APX, HAP1, REF1, Multifunctional DNA Repair Enzyme, APEX Nuclease 1, Redox factor-1, DNA-(apurinic or apyrimidinic site) lyase

Gen-ID: 79116

UniProt: P43138

Pathways: DNA Reparatur, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus