CCR2 ELISA Kit

Produktnummer ABIN5674764

Produktdetails CCR2 ELISA Kit

Target: CCR2 (Chemokine (C-C Motif) Receptor 2 (CCR2))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0061 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu CCR2

Target: CCR2 (Chemokine (C-C Motif) Receptor 2 (CCR2))

Andere Bezeichnung: Chemokine C-C-Motif Receptor 2 (CCR2) (CCR2 Produkte)

Synonyme: CC-CKR-2 ELISA Kit, CCR-2 ELISA Kit, CCR2A ELISA Kit, CCR2B ELISA Kit, CD192 ELISA Kit, CKR2 ELISA Kit, CKR2A ELISA Kit, CKR2B ELISA Kit, CMKBR2 ELISA Kit, MCP-1-R ELISA Kit, ACKR5 ELISA Kit, CKRX ELISA Kit, CRAM ELISA Kit, CRAM-A ELISA Kit, CRAM-B ELISA Kit, HCR ELISA Kit, Cc-ckr-2 ELISA Kit, Ccr2a ELISA Kit, Ccr2b ELISA Kit, Ckr2 ELISA Kit, Ckr2a ELISA Kit, Ckr2b ELISA Kit, Cmkbr2 ELISA Kit, mJe-r ELISA Kit, C-C motif chemokine receptor 2 ELISA Kit, C-C motif chemokine receptor like 2 ELISA Kit, chemokine (C-C motif) receptor 2 ELISA Kit, CCR2 ELISA Kit, CCRL2 ELISA Kit, Ccr2 ELISA Kit, LOC484790 ELISA Kit

Hintergrund: Alternative name: CD192, CC-CKR2, CCR2A, C-C CKR-2, CCR2B, CKR2-A, CKR2-B, CMKBR2, MCP1-R, Monocyte chemoattractant protein 1 receptor

Gen-ID: 12772

UniProt: P51683

Pathways: cAMP Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process