KCNJ5 ELISA Kit

Produktnummer ABIN5674765

Produktdetails KCNJ5 ELISA Kit

Target: KCNJ5 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 5 (KCNJ5))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0066 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu KCNJ5

Target: KCNJ5 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 5 (KCNJ5))

Andere Bezeichnung: Potassium Inwardly Rectifying Channel Subfamily J, Member 5 (KCNJ5) (KCNJ5 Produkte)

Synonyme: CIR ELISA Kit, GIRK4 ELISA Kit, KATP1 ELISA Kit, KIR3.4 ELISA Kit, LQT13 ELISA Kit, Kir3.4 ELISA Kit, GIRK-4 ELISA Kit, KATP-1 ELISA Kit, potassium voltage-gated channel subfamily J member 5 ELISA Kit, potassium inwardly-rectifying channel, subfamily J, member 5 ELISA Kit, KCNJ5 ELISA Kit, Kcnj5 ELISA Kit

Hintergrund: Alternative name: Kir3.4, CIR, KATP1, GIRK4, G protein-activated inward rectifier potassium channel 4, Cardiac inward rectifier, Inward rectifier K(+) channel Kir3.4, Heart KATP channel

Gen-ID: 3762

UniProt: P48544

Pathways: Notch Signalweg