NRN1 ELISA Kit

Produktnummer ABIN5674796

Produktdetails NRN1 ELISA Kit

Target: NRN1 (Neuritin 1 (NRN1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25 pg/mL - 2000 pg/mL

Untere Nachweisgrenze: 31.25 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 11.5 pg/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu NRN1

Target: NRN1 (Neuritin 1 (NRN1))

Abstract: NRN1 Produkte

Synonyme: NRN ELISA Kit, dJ380B8.2 ELISA Kit, 0710008J23Rik ELISA Kit, Cpg15 ELISA Kit, Nrn ELISA Kit, neuritin ELISA Kit, nrn1 ELISA Kit, zgc:92828 ELISA Kit, NRN1 ELISA Kit, cpg15 ELISA Kit, nrn ELISA Kit, nrn1-a ELISA Kit, nrn1-b ELISA Kit, nrn1b ELISA Kit, neuritin 1 ELISA Kit, neuritin 1a ELISA Kit, neuritin 1 S homeolog ELISA Kit, neuritin 1 L homeolog ELISA Kit, NRN1 ELISA Kit, Nrn1 ELISA Kit, nrn1a ELISA Kit, nrn1 ELISA Kit, nrn1.S ELISA Kit, nrn1.L ELISA Kit

Gen-ID: 51299

UniProt: Q9NPD7