HTR2C ELISA Kit

Produktnummer ABIN5674799

Produktdetails HTR2C ELISA Kit

Target: HTR2C (5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0064 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu HTR2C

Target: HTR2C (5-Hydroxytryptamine (serotonin) Receptor 2C (HTR2C))

Andere Bezeichnung: 5-Hydroxytryptamine Receptor 2C (HTR2C) (HTR2C Produkte)

Synonyme: 5-HT2C ELISA Kit, 5-HTR2C ELISA Kit, HTR1C ELISA Kit, 5-HT2cR ELISA Kit, 5HT1c ELISA Kit, Htr1c ELISA Kit, SR1 ELISA Kit, 5HT-1C ELISA Kit, 5HT2C ELISA Kit, 5-HT-2C ELISA Kit, 5-ht2cr ELISA Kit, si:dkey-24g18.2 ELISA Kit, 5ht2c ELISA Kit, 5HTR2C ELISA Kit, 5-hydroxytryptamine receptor 2C ELISA Kit, 5-hydroxytryptamine (serotonin) receptor 2C ELISA Kit, 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled S homeolog ELISA Kit, 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled-like 1 ELISA Kit, 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled ELISA Kit, HTR2C ELISA Kit, Htr2c ELISA Kit, htr2c.S ELISA Kit, htr2cl1 ELISA Kit, LOC407134 ELISA Kit

Hintergrund: Alternative name: 5-HT2C, HTR1C, Serotonin Receptor 2C, 5-hydroxytryptamine receptor 1C

Gen-ID: 3358

UniProt: P28335

Pathways: Inositol Metabolic Process, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour