ASS1 ELISA Kit

Produktnummer ABIN5674810

Produktdetails ASS1 ELISA Kit

Target: ASS1 (Argininosuccinate Synthase 1 (ASS1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0042 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu ASS1

Target: ASS1 (Argininosuccinate Synthase 1 (ASS1))

Andere Bezeichnung: Argininosuccinate Synthetase 1 (ASS1) (ASS1 Produkte)

Synonyme: ASS ELISA Kit, CTLN1 ELISA Kit, AA408052 ELISA Kit, Ass-1 ELISA Kit, fold ELISA Kit, ASSA ELISA Kit, Ass ELISA Kit, ass ELISA Kit, zgc:92051 ELISA Kit, wu:fb95a04 ELISA Kit, wu:fc01e08 ELISA Kit, argininosuccinate synthase 1 ELISA Kit, argininosuccinate synthetase 1 ELISA Kit, argininosuccinate synthase 1 S homeolog ELISA Kit, ASS1 ELISA Kit, Ass1 ELISA Kit, ass1.S ELISA Kit, ass1 ELISA Kit

Hintergrund: Alternative name: CTLN1, Citrulline--aspartate ligase

Gen-ID: 11898

UniProt: P16460

Pathways: Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin