NEIL3 ELISA Kit

Produktnummer ABIN5674878

Produktdetails NEIL3 ELISA Kit

Target: NEIL3 (Nei Endonuclease VIII-Like 3 (NEIL3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.125 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78.125 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 34.55 pg/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu NEIL3

Target: NEIL3 (Nei Endonuclease VIII-Like 3 (NEIL3))

Andere Bezeichnung: Nei Endonuclease VIII Like Protein 3 (NEIL3) (NEIL3 Produkte)

Synonyme: RGD1310562 ELISA Kit, NEIL3 ELISA Kit, zgc:103553 ELISA Kit, neil3 ELISA Kit, MAC12.12 ELISA Kit, MAC12_12 ELISA Kit, FGP2 ELISA Kit, FPG2 ELISA Kit, NEI3 ELISA Kit, hFPG2 ELISA Kit, hNEI3 ELISA Kit, AI449477 ELISA Kit, BC034753 ELISA Kit, C85903 ELISA Kit, nei-like DNA glycosylase 3 ELISA Kit, nei like DNA glycosylase 3 ELISA Kit, GRF zinc finger / Zinc knuckle protein ELISA Kit, nei-like DNA glycosylase 3 L homeolog ELISA Kit, nei like 3 (E. coli) ELISA Kit, Neil3 ELISA Kit, NEIL3 ELISA Kit, neil3 ELISA Kit, AT5G13920 ELISA Kit, neil3.L ELISA Kit

Hintergrund: Alternative name: FPG2, hFPG2, hNEI3, Endonuclease 8-like 3, DNA glycosylase FPG2, DNA glycosylase/AP lyase Neil3, Nei-like protein 3

Gen-ID: 55247

UniProt: Q8TAT5

Pathways: DNA Reparatur