EPHX1 ELISA Kit

Produktnummer ABIN5674887

Produktdetails EPHX1 ELISA Kit

Target: EPHX1 (Epoxide Hydrolase 1, Microsomal (Xenobiotic) (EPHX1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 28.6 pg/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu EPHX1

Target: EPHX1 (Epoxide Hydrolase 1, Microsomal (Xenobiotic) (EPHX1))

Andere Bezeichnung: Epoxide Hydrolase 1, Microsomal (EPHX1) (EPHX1 Produkte)

Synonyme: NV13267 ELISA Kit, DRB1 ELISA Kit, DSRNA-BINDING PROTEIN 1 ELISA Kit, F21M12.9 ELISA Kit, F21M12_9 ELISA Kit, HYPONASTIC LEAVES 1 ELISA Kit, AI195553 ELISA Kit, Eph-1 ELISA Kit, Eph1 ELISA Kit, mEH ELISA Kit, EPHX ELISA Kit, EPOX ELISA Kit, HYL1 ELISA Kit, MEH ELISA Kit, zgc:56126 ELISA Kit, ephx ELISA Kit, epox ELISA Kit, meh ELISA Kit, MEH8 ELISA Kit, epoxide hydrolase 1 ELISA Kit, epoxide hydrolase 1, microsomal (xenobiotic) ELISA Kit, Epoxide hydrolase 1 ELISA Kit, dsRNA-binding domain-like superfamily protein ELISA Kit, epoxide hydrolase 1, microsomal ELISA Kit, epoxide hydrolase 1, microsomal (xenobiotic) L homeolog ELISA Kit, EPHX1 ELISA Kit, Eh1 ELISA Kit, hyep ELISA Kit, HYL1 ELISA Kit, Ephx1 ELISA Kit, ephx1 ELISA Kit, ephx1.L ELISA Kit

Hintergrund: Alternative name: Epoxide hydratase, Microsomal epoxide hydrolase, Eph-1, MEH8

Gen-ID: 2052

UniProt: P07099