PDE3A ELISA Kit

Produktnummer ABIN5674894

Produktdetails PDE3A ELISA Kit

Target: PDE3A (phosphodiesterase 3A, cGMP-Inhibited (PDE3A))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0101 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu PDE3A

Target: PDE3A (phosphodiesterase 3A, cGMP-Inhibited (PDE3A))

Andere Bezeichnung: Phosphodiesterase 3A, cGMP Inhibited (PDE3A) (PDE3A Produkte)

Synonyme: PDE3A ELISA Kit, Pde3a ELISA Kit, si:dkey-48h7.2 ELISA Kit, A930022O17Rik ELISA Kit, C87899 ELISA Kit, RNPDE3A ELISA Kit, CGI-PDE ELISA Kit, CGI-PDE A ELISA Kit, CGI-PDE-A ELISA Kit, phosphodiesterase 3A ELISA Kit, phosphodiesterase 3A, cGMP-inhibited ELISA Kit, cGMP-inhibited 3',5'-cyclic phosphodiesterase A ELISA Kit, phosphodiesterase 3A, cGMP inhibited ELISA Kit, PDE3A ELISA Kit, Pde3a ELISA Kit, LOC100540515 ELISA Kit, pde3a ELISA Kit

Hintergrund: Alternative name: CGI-PDE, Cyclic GMP-inhibited phosphodiesterase A, cGMP-inhibited 3',5'-cyclic phosphodiesterase A

Gen-ID: 5139

UniProt: Q14432

Pathways: cAMP Metabolic Process