Transglutaminase 2 ELISA Kit

Produktnummer ABIN5674907

Produktdetails Transglutaminase 2 ELISA Kit

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0058 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu Transglutaminase 2

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Andere Bezeichnung: Transglutaminase 2, Tissue (TGM2) (TGM2 Produkte)

Synonyme: G-ALPHA-h ELISA Kit, GNAH ELISA Kit, TG2 ELISA Kit, TGC ELISA Kit, tgm2 ELISA Kit, zgc:101081 ELISA Kit, TgaseII ELISA Kit, TGM2 ELISA Kit, fc12b04 ELISA Kit, wu:fc12b04 ELISA Kit, zgc:136700 ELISA Kit, TGL2 ELISA Kit, G[a]h ELISA Kit, TGase2 ELISA Kit, tTG ELISA Kit, tTGas ELISA Kit, transglutaminase 2 ELISA Kit, transglutaminase 2, C polypeptide A ELISA Kit, transglutaminase 2b ELISA Kit, transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase) ELISA Kit, transglutaminase 2, C polypeptide ELISA Kit, TGM2 ELISA Kit, tgm2a ELISA Kit, Tgm2 ELISA Kit, tgm2b ELISA Kit

Hintergrund: Alternative name: tTG, TG2, C Polypeptide,Protein-Glutamine-Gamma-Glutamyltransferase, Transglutaminase C, Transglutaminase H

Gen-ID: 21817

UniProt: P21981

Pathways: Tube Formation, Thromboxane A2 Receptor Signaling