BAT3 ELISA Kit

Produktnummer ABIN5674909

Produktdetails BAT3 ELISA Kit

Target: BAT3 (Large Proline-Rich Protein BAT3 (BAT3))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0068 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu BAT3

Target: BAT3 (Large Proline-Rich Protein BAT3 (BAT3))

Andere Bezeichnung: HLA-B Associated Transcript 3 (BAT3) (BAT3 Produkte)

Synonyme: ARABIDOPSIS THALIANA BCL-2-ASSOCIATED ATHANOGENE 6 ELISA Kit, ATBAG6 ELISA Kit, BCL-2-associated athanogene 6 ELISA Kit, T3F17.11 ELISA Kit, 2410045D21Rik ELISA Kit, AA408914 ELISA Kit, BAG-6 ELISA Kit, Bat3 ELISA Kit, D17H6S52E ELISA Kit, G3 ELISA Kit, Scythe ELISA Kit, BAT3 ELISA Kit, D6S52E ELISA Kit, BCL-2-associated athanogene 6 ELISA Kit, BCL2-associated athanogene 6 ELISA Kit, BCL2 associated athanogene 6 ELISA Kit, BAG6 ELISA Kit, Bag6 ELISA Kit

Hintergrund: Alternative name: BAG6, Protein G3, Protein Scythe, BCL2-associated athanogene 6, BAG family molecular chaperone regulator 6, Large proline-rich protein BAG6

Gen-ID: 94342

UniProt: Q6MG49