RNLS ELISA Kit

Produktnummer ABIN5674910

Produktdetails RNLS ELISA Kit

Target: RNLS (Renalase, FAD-Dependent Amine Oxidase (RNLS))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Untere Nachweisgrenze: 1.56 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Sensitivität: 0.69 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu RNLS

Target: RNLS (Renalase, FAD-Dependent Amine Oxidase (RNLS))

Andere Bezeichnung: Renalase (RNLS) (RNLS Produkte)

Synonyme: zgc:92286 ELISA Kit, C10orf59 ELISA Kit, RENALASE ELISA Kit, 6530404N21Rik ELISA Kit, AI452315 ELISA Kit, AW060440 ELISA Kit, RGD1309804 ELISA Kit, renalase, FAD dependent amine oxidase ELISA Kit, renalase, FAD-dependent amine oxidase ELISA Kit, RNLS ELISA Kit, rnls ELISA Kit, Rnls ELISA Kit

Hintergrund: Alternative name: MAO-C, C10orf60, FAD-Dependent Amine Oxidase, Monoamine oxidase-C, alpha-NAD(P)H oxidase/anomerase

Gen-ID: 67795

UniProt: A7RDN6