NME1 ELISA Kit

Produktnummer ABIN5674914

Produktdetails NME1 ELISA Kit

Target: NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Sensitivität: 0053 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu NME1

Target: NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))

Andere Bezeichnung: Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) (NME1 Produkte)

Synonyme: AWD ELISA Kit, GAAD ELISA Kit, NB ELISA Kit, NBS ELISA Kit, NDKA ELISA Kit, NDPK-A ELISA Kit, NDPKA ELISA Kit, NM23 ELISA Kit, NM23-H1 ELISA Kit, AL024257 ELISA Kit, NM23-M1 ELISA Kit, NM23A ELISA Kit, NDPK-Z1 ELISA Kit, NM23-B ELISA Kit, NM23-Z1 ELISA Kit, ndpkz1 ELISA Kit, nm23b ELISA Kit, nme1 ELISA Kit, nme2 ELISA Kit, nme2b1 ELISA Kit, NME1 ELISA Kit, NME1-NME2 ELISA Kit, NM23-C1 ELISA Kit, ndpk-b ELISA Kit, ndpkb ELISA Kit, nm23-h2 ELISA Kit, nm23ndk ELISA Kit, puf ELISA Kit, NBR-A ELISA Kit, NBR-B ELISA Kit, NME1-1 ELISA Kit, nm23ndk-a ELISA Kit, NDK I ELISA Kit, NDPK I ELISA Kit, T30A10.80 ELISA Kit, T30A10_80 ELISA Kit, NDP kinase I ELISA Kit, NME/NM23 nucleoside diphosphate kinase 1 ELISA Kit, NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 ELISA Kit, non-metastatic cells 1, protein (NM23A) expressed in ELISA Kit, NME/NM23 nucleoside diphosphate kinase 2 S homeolog ELISA Kit, NME/NM23 nucleoside diphosphate kinase 2 L homeolog ELISA Kit, nucleoside diphosphate kinase ELISA Kit, nucleoside diphosphate kinase 1 ELISA Kit, NME1 ELISA Kit, Nme1 ELISA Kit, nme2b.1 ELISA Kit, nme2.S ELISA Kit, nme2.L ELISA Kit, LOC547870 ELISA Kit, NDPK1 ELISA Kit, LOC107790563 ELISA Kit

Hintergrund: Alternative name: AWD, GAAD, NDPKA, NM23-H1, Nucleoside diphosphate kinase A, Granzyme A-activated DNase, Metastasis inhibition factor nm23, Tumor metastatic process-associated protein

Gen-ID: 18102

UniProt: P15532

Pathways: Apoptose, Nucleotide Phosphorylation, Carbohydrate Homeostasis, Ribonucleoside Biosynthetic Process