TREH ELISA Kit

Produktnummer ABIN5674939

Produktdetails TREH ELISA Kit

Target: TREH (Trehalase (Brush-Border Membrane Glycoprotein) (TREH))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.625 ng/mL - 40 ng/mL

Untere Nachweisgrenze: 0.625 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0224 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu TREH

Target: TREH (Trehalase (Brush-Border Membrane Glycoprotein) (TREH))

Andere Bezeichnung: Trehalase (TREH) (TREH Produkte)

Synonyme: 87D3T ELISA Kit, CG9364 ELISA Kit, Dmel\\CG9364 ELISA Kit, ESTS:87D3T ELISA Kit, TRE ELISA Kit, TREH ELISA Kit, Tre ELISA Kit, anon-EST:Posey159 ELISA Kit, anon-sts38 ELISA Kit, GB16316 ELISA Kit, TREA ELISA Kit, 2210412M19Rik ELISA Kit, Trehalase ELISA Kit, trehalase ELISA Kit, trehalase (brush-border membrane glycoprotein) ELISA Kit, Treh ELISA Kit, LOC693027 ELISA Kit, LOC410795 ELISA Kit, TREH ELISA Kit

Hintergrund: Alternative name: TRE, TREA, Brush Border Membrane Glycoprotein, Alpha,Alpha-Trehalase, Alpha,Alpha-Trehalose Glucohydrolase

Gen-ID: 11181

UniProt: O43280