SESN1 ELISA Kit

Produktnummer ABIN5674941

Produktdetails SESN1 ELISA Kit

Target: SESN1 (Sestrin 1 (SESN1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0064 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu SESN1

Target: SESN1 (Sestrin 1 (SESN1))

Abstract: SESN1 Produkte

Synonyme: zgc:91970 ELISA Kit, sestrin-1 ELISA Kit, SESN1 ELISA Kit, XPA26 ELISA Kit, pa26 ELISA Kit, sest1 ELISA Kit, PA26 ELISA Kit, SEST1 ELISA Kit, 1110002G11Rik ELISA Kit, AU044290 ELISA Kit, Pa26 ELISA Kit, Sest1 ELISA Kit, sesn1-A ELISA Kit, sestrin 1 ELISA Kit, sestrin 1 L homeolog ELISA Kit, SESN1 ELISA Kit, sesn1 ELISA Kit, Sesn1 ELISA Kit, sesn1.L ELISA Kit

Hintergrund: Alternative name: SEST1, PA26, p53-regulated protein PA26

Gen-ID: 27244

UniProt: Q9Y6P5