JKAMP ELISA Kit
JKAMP
Reaktivität: Human
Colorimetric
Sandwich ELISA
0.781 ng/mL - 50 ng/mL
Tissue Homogenate
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- Target Alle JKAMP ELISA Kits anzeigen
- JKAMP (JNK1/MAPK8-Associated Membrane Protein (JKAMP))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.781 ng/mL - 50 ng/mL
- Untere Nachweisgrenze
- 0.781 ng/mL
- Applikation
- ELISA
- Proben
- Tissue Homogenate
- Analytische Methode
- Quantitative
- Sensitivität
- 0.35 ng/mL
- Bestandteile
-
Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - Benötigtes Material
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Top Product
- Discover our top product JKAMP ELISA Kit
-
JNK1/MAPK8-Associated Membrane Protein (JKAMP) ELISA Kit
JKAMP Reaktivität: Ratte Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Testdauer
- 1 - 4.5 h
- Plattentyp
- Pre-coated
- Protokoll
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- 4 °C
- Haltbarkeit
- 12 months
-
- Target Alle JKAMP ELISA Kits anzeigen
- JKAMP (JNK1/MAPK8-Associated Membrane Protein (JKAMP))
- Andere Bezeichnung
- JNK1/MAPK8 Associated Membrane Protein (JKAMP) (JKAMP Produkte)
- Synonyme
- C14orf100 ELISA Kit, HSPC213 ELISA Kit, HSPC327 ELISA Kit, JAMP ELISA Kit, 1200003C05Rik ELISA Kit, 2310047L11Rik ELISA Kit, Jamp ELISA Kit, RGD1308917 ELISA Kit, JNK1/MAPK8 associated membrane protein ELISA Kit, JNK1/MAPK8-associated membrane protein ELISA Kit, JKAMP ELISA Kit, Jkamp ELISA Kit
- Hintergrund
- Alternative name: JAMP, C14orf100, Jun N-Terminal Kinase 1-Associated Membrane Protein, Medulloblastoma antigen MU-MB-50.4
- Gen-ID
- 51528
- UniProt
- Q9P055
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