SDHA ELISA Kit

Produktnummer ABIN5674947

Produktdetails SDHA ELISA Kit

Target: SDHA (Succinate Dehydrogenase Complex, Subunit A, Flavoprotein (Fp) (SDHA))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.125 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78.125 pg/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 33.2 pg/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu SDHA

Target: SDHA (Succinate Dehydrogenase Complex, Subunit A, Flavoprotein (Fp) (SDHA))

Andere Bezeichnung: Succinate Dehydrogenase Complex Subunit A (SDHA) (SDHA Produkte)

Synonyme: CG17246 ELISA Kit, Dmel\\CG17246 ELISA Kit, Scs-fp ELISA Kit, Sdh ELISA Kit, Sdha ELISA Kit, scs-fp ELISA Kit, sdh ELISA Kit, CMD1GG ELISA Kit, FP ELISA Kit, PGL5 ELISA Kit, SDH1 ELISA Kit, SDH2 ELISA Kit, SDHF ELISA Kit, 1500032O14Rik ELISA Kit, 2310034D06Rik ELISA Kit, 4921513A11 ELISA Kit, C81073 ELISA Kit, im:7141001 ELISA Kit, zgc:56051 ELISA Kit, Fp ELISA Kit, sdha-b ELISA Kit, Succinate dehydrogenase, subunit A (flavoprotein) ELISA Kit, succinate dehydrogenase complex flavoprotein subunit A ELISA Kit, succinate dehydrogenase complex, subunit A, flavoprotein (Fp) ELISA Kit, succinate dehydrogenase complex subunit A, flavoprotein (Fp) S homeolog ELISA Kit, SdhA ELISA Kit, SDHA ELISA Kit, Sdha ELISA Kit, sdha ELISA Kit, sdha.S ELISA Kit

Hintergrund: Alternative name: SDH2, SDHF, FP, Flavoprotein, Flavoprotein subunit of complex II, Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

Gen-ID: 6389

UniProt: P31040