ALDH7A1 ELISA Kit

Produktnummer ABIN5674953

Produktdetails ALDH7A1 ELISA Kit

Target: ALDH7A1 (Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.781 ng/mL - 50 ng/mL

Untere Nachweisgrenze: 0.781 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0.29 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu ALDH7A1

Target: ALDH7A1 (Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1))

Abstract: ALDH7A1 Produkte

Synonyme: atq1 ELISA Kit, ALDH7A1 ELISA Kit, ATQ1 ELISA Kit, EPD ELISA Kit, PDE ELISA Kit, antiquitin ELISA Kit, ATQ ELISA Kit, wu:fi34d12 ELISA Kit, wu:fi35e06 ELISA Kit, Atq1 ELISA Kit, D18Wsu181e ELISA Kit, aldehyde dehydrogenase 7 family member A1 ELISA Kit, aldehyde dehydrogenase 7 family member A1 L homeolog ELISA Kit, aldehyde dehydrogenase 7 family, member A1 ELISA Kit, aldehyde dehydrogenase family 7, member A1 ELISA Kit, ALDH7A1 ELISA Kit, aldh7a1 ELISA Kit, aldh7a1.L ELISA Kit, Aldh7a1 ELISA Kit

Hintergrund: Alternative name: ATQ1, PDE, EPD, Antiquitin, Alpha-Aminoadipic Semialdehyde Dehydrogenase, Delta1-Piperideine-6-Carboxylate Dehydrogenease, P6c Dehydrogenase

Gen-ID: 501

UniProt: P49419

Pathways: Sensory Perception of Sound