PPARA ELISA Kit

Produktnummer ABIN5690760

Produktdetails PPARA ELISA Kit

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: DNA-Binding ELISA

Applikation: ELISA

Verwendungszweck:  Human PPAR-alpha Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical PPAR-alpha binding sequences to semi-quantitatively detect active PPAR-alpha in lysates or nuclear extracts. 

Proben: Cell Lysate, Nuclear Extract

Analytische Methode: Semi-Quantitative

Spezifität: The olionucleotide/antibody pair provided in this kit recognizes human PPAR-alpha in whole lysates and nuclear extracts.

Produktmerkmale:

• Specific transcription factor-DNA binding assay
• Perfect alternative to EMSA
• Easy to perform in an ELISA format
• Non-radioactive assay
• High throughput (96 well plate format)
• Assay can be completed within 5 hours

Bestandteile:

• 96-well Strip Microplate pre-coated with DNA probes
• DNA Binding Buffer
• Positive Control Sample
• Specific Competitor DNA probe
• Non-specific Competitor DNA probe
• Assay Reagent
• DTT
• Wash Buffer
• Primary Antibody
• HRP-conjugated Secondary Antibody
• TMB One-Step Substrate Reagent
• Stop Solution

Benötigtes Material:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions Absorbent paper
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared HRP-secondary antibody to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 6 months

Details zu PPARA

Target: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Andere Bezeichnung: PPAR-alpha (PPARA Produkte)

Synonyme: NR1C1 ELISA Kit, PPAR ELISA Kit, PPARalpha ELISA Kit, hPPAR ELISA Kit, LOC100136415 ELISA Kit, PPARALPHA ELISA Kit, nr1c1 ELISA Kit, xPPARalpha ELISA Kit, xppara ELISA Kit, 4933429D07Rik ELISA Kit, AW742785 ELISA Kit, Nr1c1 ELISA Kit, PPAR-alpha ELISA Kit, Ppar ELISA Kit, peroxisome proliferator activated receptor alpha ELISA Kit, peroxisome proliferator-activated receptor alpha ELISA Kit, peroxisome proliferator activated receptor alpha L homeolog ELISA Kit, PPARA ELISA Kit, LOC100136415 ELISA Kit, ppara.L ELISA Kit, Ppara ELISA Kit, ppara ELISA Kit

Gen-ID: 5465

UniProt: Q07869

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C