ACP6 ELISA Kit

Produktnummer ABIN5690814

Produktdetails ACP6 ELISA Kit

Target: ACP6 (Acid Phosphatase 6, Lysophosphatidic (ACP6))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human ACP6 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human ACP6.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 6 months

Details zu ACP6

Target: ACP6 (Acid Phosphatase 6, Lysophosphatidic (ACP6))

Andere Bezeichnung: ACP6 (ACP6 Produkte)

Synonyme: ACP6 ELISA Kit, im:7147584 ELISA Kit, zgc:172268 ELISA Kit, MGC146066 ELISA Kit, ACPL1 ELISA Kit, LPAP ELISA Kit, PACPL1 ELISA Kit, 5730559A09Rik ELISA Kit, AU022842 ELISA Kit, mPACPL1 ELISA Kit, acid phosphatase 6, lysophosphatidic ELISA Kit, acid phosphatase 6, lysophosphatidic S homeolog ELISA Kit, ACP6 ELISA Kit, acp6 ELISA Kit, acp6.S ELISA Kit, Acp6 ELISA Kit

Gen-ID: 51205

UniProt: Q9NPH0