C9orf3 ELISA Kit

Produktnummer ABIN5690820

Produktdetails C9orf3 ELISA Kit

Target: C9orf3 (Chromosome 9 Open Reading Frame 3 (C9orf3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human Aminopeptidase O ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human Aminopeptidase O.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 6 months

Details zu C9orf3

Target: C9orf3 (Chromosome 9 Open Reading Frame 3 (C9orf3))

Andere Bezeichnung: Aminopeptidase O (C9orf3 Produkte)

Synonyme: apo ELISA Kit, ap-o ELISA Kit, aopep ELISA Kit, MGC68599 ELISA Kit, Aopep ELISA Kit, Apo ELISA Kit, RGD1309592 ELISA Kit, AOPEP ELISA Kit, AP-O ELISA Kit, APO ELISA Kit, C90RF3 ELISA Kit, ONPEP ELISA Kit, 2300006M17Rik ELISA Kit, chromosome 9 open reading frame 3 L homeolog ELISA Kit, chromosome 9 open reading frame 3 ELISA Kit, aminopeptidase O ELISA Kit, RIKEN cDNA 2010111I01 gene ELISA Kit, c9orf3.L ELISA Kit, c9orf3 ELISA Kit, Npepo ELISA Kit, C9orf3 ELISA Kit, 2010111I01Rik ELISA Kit

Gen-ID: 84909

UniProt: Q8N6M6