Crk ELISA Kit

Produktnummer ABIN5690859

Produktdetails Crk ELISA Kit

Target: Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human Crk ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human Crk.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 6 months

Details zu Crk

Target: Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))

Andere Bezeichnung: Crk (CRK Produkte)

Synonyme: CG1587 ELISA Kit, CRK ELISA Kit, D-CRK ELISA Kit, D-Crk ELISA Kit, DCrk ELISA Kit, Dcrk ELISA Kit, Dmel\\CG1587 ELISA Kit, crk ELISA Kit, dCRK ELISA Kit, dCrk ELISA Kit, fb34h10 ELISA Kit, fc05a01 ELISA Kit, fi19g05 ELISA Kit, wu:fb34h10 ELISA Kit, wu:fc05a01 ELISA Kit, wu:fc54a04 ELISA Kit, wu:fi19g05 ELISA Kit, zgc:100936 ELISA Kit, CRKII ELISA Kit, p38 ELISA Kit, Crko ELISA Kit, crk2 ELISA Kit, crkII ELISA Kit, v-crk ELISA Kit, P38C-CRK ELISA Kit, Crk-I ELISA Kit, Crk-II ELISA Kit, Crk-III ELISA Kit, Crk3 ELISA Kit, CrkIII ELISA Kit, c-Crk ELISA Kit, Crk oncogene ELISA Kit, v-crk avian sarcoma virus CT10 oncogene homolog ELISA Kit, CRK proto-oncogene, adaptor protein ELISA Kit, v-crk avian sarcoma virus CT10 oncogene homolog L homeolog ELISA Kit, v-crk sarcoma virus CT10 oncogene homolog ELISA Kit, Crk ELISA Kit, crk ELISA Kit, CRK ELISA Kit, crk.L ELISA Kit

Gen-ID: 1398

UniProt: P46108

Pathways: Neurotrophin Signalübertragung, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor