FOXM1 ELISA Kit

Produktnummer ABIN5690876

Produktdetails FOXM1 ELISA Kit

Target: FOXM1 (Forkhead Box M1 (FOXM1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human FoxM1/FKHL16 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human FoxM1.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 6 months

Details zu FOXM1

Target: FOXM1 (Forkhead Box M1 (FOXM1))

Andere Bezeichnung: FoxM1 (FKHL16 (FOXM1 Produkte)

Synonyme: FKHL16 ELISA Kit, FOXM1B ELISA Kit, HFH-11 ELISA Kit, HFH11 ELISA Kit, HNF-3 ELISA Kit, INS-1 ELISA Kit, MPHOSPH2 ELISA Kit, MPP-2 ELISA Kit, MPP2 ELISA Kit, PIG29 ELISA Kit, TGT3 ELISA Kit, TRIDENT ELISA Kit, FOXM1 ELISA Kit, AA408308 ELISA Kit, AW554517 ELISA Kit, BB238854 ELISA Kit, Fkh16 ELISA Kit, Foxm1b ELISA Kit, HFH-11B ELISA Kit, Mpm2 ELISA Kit, WIN ELISA Kit, Trident ELISA Kit, fkhl16 ELISA Kit, foxm1l ELISA Kit, hfh-11 ELISA Kit, mpp2 ELISA Kit, zgc:63854 ELISA Kit, forkhead box M1 ELISA Kit, forkhead box M1 S homeolog ELISA Kit, FOXM1 ELISA Kit, Foxm1 ELISA Kit, foxm1 ELISA Kit, foxm1.S ELISA Kit

Gen-ID: 2305

UniProt: Q08050

Pathways: Positive Regulation of Response to DNA Damage Stimulus