GLB1 ELISA Kit

Produktnummer ABIN5690881

Produktdetails GLB1 ELISA Kit

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human GLB1/Beta-galactosidase ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human GLB1.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 6 months

Details zu GLB1

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Andere Bezeichnung: GLB1 (Beta-galactosidase (GLB1 Produkte)

Synonyme: EBP ELISA Kit, ELNR1 ELISA Kit, MPS4B ELISA Kit, DER ELISA Kit, XR ELISA Kit, 0610038K04Rik ELISA Kit, 1810027P18Rik ELISA Kit, glb ELISA Kit, CG9092 ELISA Kit, Dmel\\CG9092 ELISA Kit, beta-GAL ELISA Kit, beta-Gal-1 ELISA Kit, beta-gal ELISA Kit, gal ELISA Kit, lacZ-1 ELISA Kit, AW125515 ELISA Kit, Bge ELISA Kit, Bgl ELISA Kit, Bgl-e ELISA Kit, Bgl-s ELISA Kit, Bgl-t ELISA Kit, Bgs ELISA Kit, Bgt ELISA Kit, C130097A14Rik ELISA Kit, BGAL ELISA Kit, zgc:110823 ELISA Kit, ECK0341 ELISA Kit, JW0335 ELISA Kit, GLB ELISA Kit, galactosidase beta 1 ELISA Kit, dicarbonyl and L-xylulose reductase ELISA Kit, dicarbonyl L-xylulose reductase ELISA Kit, beta-D-galactosidase ELISA Kit, beta-galactosidase ELISA Kit, beta galactosidase ELISA Kit, galactosidase, beta 1 ELISA Kit, beta-galactosidase-like ELISA Kit, GLB1 ELISA Kit, DCXR ELISA Kit, Dcxr ELISA Kit, lacZ ELISA Kit, SSP_RS00525 ELISA Kit, lacH ELISA Kit, ECL_03691 ELISA Kit, Gal ELISA Kit, Glb1 ELISA Kit, glb1 ELISA Kit, VV2_1327 ELISA Kit, LOC100056371 ELISA Kit

Gen-ID: 2720

UniProt: P16278

Pathways: Glycosaminoglycan Metabolic Process