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ILKAP ELISA Kit

ILKAP Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN5690894
  • Target Alle ILKAP ELISA Kits anzeigen
    ILKAP (Integrin-Linked Kinase-Associated Serine/threonine Phosphatase 2C (ILKAP))
    Reaktivität
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human ILKAP ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes Human ILKAP.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Haltbarkeit
    6 months
  • Target Alle ILKAP ELISA Kits anzeigen
    ILKAP (Integrin-Linked Kinase-Associated Serine/threonine Phosphatase 2C (ILKAP))
    Andere Bezeichnung
    ILKAP (ILKAP Produkte)
    Synonyme
    MGC84595 ELISA Kit, MGC107938 ELISA Kit, ILKAP ELISA Kit, ILKAP2 ELISA Kit, ILKAP3 ELISA Kit, PP2C-DELTA ELISA Kit, 0710007A14Rik ELISA Kit, 1600009O09Rik ELISA Kit, ILK associated serine/threonine phosphatase ELISA Kit, ILK associated serine/threonine phosphatase L homeolog ELISA Kit, integrin-linked kinase-associated serine/threonine phosphatase ELISA Kit, integrin-linked kinase-associated serine/threonine phosphatase 2C ELISA Kit, ILKAP ELISA Kit, ilkap.L ELISA Kit, ilkap ELISA Kit, Ilkap ELISA Kit
    Gen-ID
    80895
    UniProt
    Q9H0C8
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