IMPDH1 ELISA Kit

Produktnummer ABIN5690895

Produktdetails IMPDH1 ELISA Kit

Target: IMPDH1 (Inosine 5'-Phosphate Dehydrogenase 1 (IMPDH1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human IMPDH1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human IMPDH1.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 6 months

Details zu IMPDH1

Target: IMPDH1 (Inosine 5'-Phosphate Dehydrogenase 1 (IMPDH1))

Andere Bezeichnung: IMPDH1 (IMPDH1 Produkte)

Synonyme: IMPD ELISA Kit, IMPD1 ELISA Kit, LCA11 ELISA Kit, RP10 ELISA Kit, sWSS2608 ELISA Kit, B930086D20Rik ELISA Kit, IMPD 1 ELISA Kit, IMPDH 1 ELISA Kit, D3 ELISA Kit, IMPD 1b ELISA Kit, IMPDH 1b ELISA Kit, IMPDH1 ELISA Kit, id:ibd5035 ELISA Kit, si:dkey-31f5.7 ELISA Kit, wu:fa09h11 ELISA Kit, wu:fa99c03 ELISA Kit, zgc:113446 ELISA Kit, imp ELISA Kit, imp1 ELISA Kit, imp2 ELISA Kit, IMPD 1a ELISA Kit, IMPDH 1a ELISA Kit, zgc:91911 ELISA Kit, inosine monophosphate dehydrogenase 1 ELISA Kit, inosine-5'-monophosphate dehydrogenase 1 ELISA Kit, IMP (inosine 5'-monophosphate) dehydrogenase 1b ELISA Kit, IMP (inosine 5'-monophosphate) dehydrogenase 1 ELISA Kit, IMP (inosine 5'-monophosphate) dehydrogenase 1a ELISA Kit, IMPDH1 ELISA Kit, Impdh1 ELISA Kit, LOC100442769 ELISA Kit, impdh1 ELISA Kit, impdh1b ELISA Kit, impdh1.L ELISA Kit, impdh1a ELISA Kit

Gen-ID: 3614

UniProt: P20839

Pathways: Ribonucleoside Biosynthetic Process