IRF2 ELISA Kit

Produktnummer ABIN5690898

Produktdetails IRF2 ELISA Kit

Target: IRF2 (Interferon Regulatory Factor 2 (IRF2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human IRF-2 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human IRF-2.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 6 months

Details zu IRF2

Target: IRF2 (Interferon Regulatory Factor 2 (IRF2))

Andere Bezeichnung: IRF-2 (IRF2 Produkte)

Synonyme: IRF-2 ELISA Kit, 9830146E22Rik ELISA Kit, AI646973 ELISA Kit, Irf-2 ELISA Kit, irf2b ELISA Kit, zgc:103451 ELISA Kit, irf-2 ELISA Kit, wu:fc74h04 ELISA Kit, zgc:76951 ELISA Kit, interferon regulatory factor 2 ELISA Kit, interferon regulatory factor 2 L homeolog ELISA Kit, interferon regulatory factor 2a ELISA Kit, IRF2 ELISA Kit, Irf2 ELISA Kit, irf2 ELISA Kit, irf2.L ELISA Kit, irf2a ELISA Kit

Gen-ID: 3660

UniProt: P14316