Myogenin ELISA Kit

Produktnummer ABIN5690915

Produktdetails Myogenin ELISA Kit

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human Myogenin ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human Myogenin.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 6 months

Details zu Myogenin

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Andere Bezeichnung: Myogenin (MYOG Produkte)

Synonyme: cb553 ELISA Kit, zgc:100763 ELISA Kit, LOC446037 ELISA Kit, myf4 ELISA Kit, Xmyog ELISA Kit, myogenin ELISA Kit, LOC100136471 ELISA Kit, LOC100224062 ELISA Kit, LOC100303673 ELISA Kit, MYF4 ELISA Kit, bHLHc3 ELISA Kit, myo ELISA Kit, myf-4 ELISA Kit, myogenin ELISA Kit, myogenin (myogenic factor 4) ELISA Kit, MYOG ELISA Kit, myog ELISA Kit, LOC100303673 ELISA Kit, Myog ELISA Kit

Gen-ID: 4656

UniProt: P15173

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development