NFATC3 ELISA Kit

Produktnummer ABIN5690919

Produktdetails NFATC3 ELISA Kit

Target: NFATC3 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 3 (NFATC3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human NF-ATc3 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human NF-ATc3.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 6 months

Details zu NFATC3

Target: NFATC3 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 3 (NFATC3))

Andere Bezeichnung: NF-ATc3 (NFATC3 Produkte)

Synonyme: NFATC3 ELISA Kit, NFATx ELISA Kit, nf-at ELISA Kit, nfat4 ELISA Kit, nfatx ELISA Kit, C80703 ELISA Kit, D8Ertd281e ELISA Kit, NFAT4 ELISA Kit, NFATX ELISA Kit, nuclear factor of activated T-cells 3 ELISA Kit, nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3 ELISA Kit, nuclear factor of activated T-cells 3 S homeolog ELISA Kit, NFATC3 ELISA Kit, nfatc3 ELISA Kit, Nfatc3 ELISA Kit, nfatc3.S ELISA Kit

Gen-ID: 4775

UniProt: Q12968

Pathways: RTK Signalweg, WNT Signalweg, Fc-epsilon Rezeptor Signalübertragung, Chromatin Binding