Parkin ELISA Kit

Produktnummer ABIN5690927

Produktdetails Parkin ELISA Kit

Target: Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human Parkin ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human Parkin.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 6 months

Details zu Parkin

Target: Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))

Andere Bezeichnung: Parkin (PARK2 Produkte)

Synonyme: CG10523 ELISA Kit, Dmel\\CG10523 ELISA Kit, Dpark ELISA Kit, SD01679 ELISA Kit, dpk ELISA Kit, AR-JP ELISA Kit, LPRS2 ELISA Kit, PDJ ELISA Kit, PRKN ELISA Kit, Park ELISA Kit, Prkn ELISA Kit, si:ch211-123f21.1 ELISA Kit, zgc:112390 ELISA Kit, pdr-1 ELISA Kit, PARK2 ELISA Kit, parkin ELISA Kit, parkin RBR E3 ubiquitin protein ligase ELISA Kit, Parkinson disease (autosomal recessive, juvenile) 2, parkin ELISA Kit, park ELISA Kit, PRKN ELISA Kit, Prkn ELISA Kit, prkn ELISA Kit, CpipJ_CPIJ014867 ELISA Kit, Park2 ELISA Kit

Gen-ID: 5071

UniProt: O60260

Pathways: Autophagie, Ubiquitin Proteasome Pathway