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PGPEP1 ELISA Kit

PGPEP1 Reaktivität: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN5690932
  • Target Alle PGPEP1 ELISA Kits anzeigen
    PGPEP1 (Pyroglutamyl-Peptidase I (PGPEP1))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human PGPEP-1 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes Human PGPEP-1.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C
    Haltbarkeit
    6 months
  • Target Alle PGPEP1 ELISA Kits anzeigen
    PGPEP1 (Pyroglutamyl-Peptidase I (PGPEP1))
    Andere Bezeichnung
    PGPEP-1 (PGPEP1 Produkte)
    Synonyme
    PGP ELISA Kit, PGP-I ELISA Kit, PGPI ELISA Kit, Pcp ELISA Kit, 2810003H13Rik ELISA Kit, Gm19458 ELISA Kit, PAP-I ELISA Kit, PAPI ELISA Kit, pyroglutamyl-peptidase I ELISA Kit, Pyroglutamyl-peptidase I ELISA Kit, Fnod_0998 ELISA Kit, Tlet_1256 ELISA Kit, Franean1_3440 ELISA Kit, Sked_37410 ELISA Kit, Arch_1014 ELISA Kit, Olsu_1567 ELISA Kit, Bcell_2984 ELISA Kit, Deima_2337 ELISA Kit, Spico_0458 ELISA Kit, Poras_1572 ELISA Kit, PGPEP1 ELISA Kit, Pgpep1 ELISA Kit
    Gen-ID
    54858
    UniProt
    Q9NXJ5
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