PPP1R2 ELISA Kit
PPP1R2
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN5690938
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Target
Alle PPP1R2 Produkte
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PPP1R2
(Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 2 (PPP1R2))
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Reaktivität
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Human
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Nachweismethode
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Colorimetric
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Methodentyp
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Sandwich ELISA
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Applikation
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ELISA
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Verwendungszweck
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Human PP1 Inhibitor-2/IPP-2 ELISA Kit.
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Proben
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytische Methode
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Quantitative
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Spezifität
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This ELISA antibody pair recognizes Human PP1 Inhibitor-2.
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Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Plattentyp
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Pre-coated
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Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Lagerung
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4 °C
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Haltbarkeit
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6 months
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Target
Alle PPP1R2 Produkte
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PPP1R2
(Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 2 (PPP1R2))
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Andere Bezeichnung
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PP1 Inhibitor-2 (IPP-2 (PPP1R2 Produkte)
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Synonyme
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DDBDRAFT_0167872 ELISA Kit, DDBDRAFT_0233418 ELISA Kit, DDB_0167872 ELISA Kit, DDB_0233418 ELISA Kit, IPP-2 ELISA Kit, IPP2 ELISA Kit, 0610025N14Rik ELISA Kit, 2310007G06Rik ELISA Kit, 4930440J04Rik ELISA Kit, 5430408E15Rik ELISA Kit, D16Ertd248e ELISA Kit, protein phosphatase inhibitor 2 ELISA Kit, protein phosphatase 1 regulatory inhibitor subunit 2 ELISA Kit, protein phosphatase 1, regulatory (inhibitor) subunit 2 ELISA Kit, dpiA ELISA Kit, Tsp_08270 ELISA Kit, PPP1R2 ELISA Kit, Ppp1r2 ELISA Kit
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Gen-ID
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5504
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UniProt
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P41236
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Pathways
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PI3K-Akt Signalweg, Cellular Glucan Metabolic Process
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