PTPN11 ELISA Kit
PTPN11
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN5690950
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Target
Alle PTPN11 ELISA Kits anzeigen
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PTPN11
(Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))
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Reaktivität
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Human
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Nachweismethode
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Colorimetric
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Methodentyp
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Sandwich ELISA
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Applikation
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ELISA
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Verwendungszweck
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Human SHP-2 ELISA Kit.
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Proben
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytische Methode
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Quantitative
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Spezifität
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This ELISA antibody pair recognizes Human SHP-2.
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Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Top Product
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Discover our top product PTPN11 ELISA Kit
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Plattentyp
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Pre-coated
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Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Lagerung
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4 °C
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Haltbarkeit
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6 months
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Target
Alle PTPN11 ELISA Kits anzeigen
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PTPN11
(Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))
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Andere Bezeichnung
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SHP-2 (PTPN11 Produkte)
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Synonyme
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BPTP3 ELISA Kit, CFC ELISA Kit, NS1 ELISA Kit, PTP-1D ELISA Kit, PTP2C ELISA Kit, SH-PTP2 ELISA Kit, SH-PTP3 ELISA Kit, SHP2 ELISA Kit, 2700084A17Rik ELISA Kit, AW536184 ELISA Kit, PTP1D ELISA Kit, SAP-2 ELISA Kit, SHP-2 ELISA Kit, Shp2 ELISA Kit, Syp ELISA Kit, SYP ELISA Kit, bptp3 ELISA Kit, cfc ELISA Kit, ns1 ELISA Kit, ptp-2 ELISA Kit, ptp2c ELISA Kit, ptpn11 ELISA Kit, ptpn11-a ELISA Kit, ptpn11-b ELISA Kit, shp-2 ELISA Kit, shp2 ELISA Kit, fa14b09 ELISA Kit, wu:fa14b09 ELISA Kit, wu:fi24f03 ELISA Kit, zgc:55388 ELISA Kit, zgc:63553 ELISA Kit, protein tyrosine phosphatase, non-receptor type 11 ELISA Kit, protein tyrosine phosphatase, non-receptor type 11 S homeolog ELISA Kit, protein tyrosine phosphatase, non-receptor type 11, a ELISA Kit, protein tyrosine phosphatase, non-receptor type 11, b ELISA Kit, PTPN11 ELISA Kit, Ptpn11 ELISA Kit, ptpn11.S ELISA Kit, ptpn11a ELISA Kit, ptpn11b ELISA Kit
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Substanzklasse
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Viral Protein
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Gen-ID
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5781
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UniProt
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Q06124
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Pathways
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JAK-STAT Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, Warburg Effekt
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