TSC1 ELISA Kit

Produktnummer ABIN5690964

Produktdetails TSC1 ELISA Kit

Target: TSC1 (Tuberous Sclerosis 1 (TSC1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human TSC1/Hamartin ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human TSC1.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 6 months

Details zu TSC1

Target: TSC1 (Tuberous Sclerosis 1 (TSC1))

Andere Bezeichnung: TSC1 (Hamartin (TSC1 Produkte)

Synonyme: LAM ELISA Kit, TSC ELISA Kit, hamartin ELISA Kit, mKIAA0243 ELISA Kit, tsc1 ELISA Kit, wu:fc38b04 ELISA Kit, fa99f04 ELISA Kit, wu:fa99f04 ELISA Kit, zgc:63657 ELISA Kit, TSC complex subunit 1 ELISA Kit, tuberous sclerosis 1 ELISA Kit, TSC complex subunit 1b ELISA Kit, TSC complex subunit 1a ELISA Kit, TSC1 ELISA Kit, Tsc1 ELISA Kit, tsc1b ELISA Kit, tsc1 ELISA Kit, tsc1a ELISA Kit

Gen-ID: 7248

UniProt: Q92574

Pathways: RTK Signalweg, AMPK Signaling, Regulation of Cell Size, Tube Formation