UFM1 ELISA Kit

Produktnummer ABIN5690967

Produktdetails UFM1 ELISA Kit

Target: UFM1 (Ubiquitin-Fold Modifier 1 (UFM1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human UFM1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human UFM1.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 6 months

Details zu UFM1

Target: UFM1 (Ubiquitin-Fold Modifier 1 (UFM1))

Andere Bezeichnung: UFM1 (UFM1 Produkte)

Synonyme: T32E8.4 ELISA Kit, T32E8_4 ELISA Kit, BM-002 ELISA Kit, C13orf20 ELISA Kit, bA131P10.1 ELISA Kit, 1810045K17Rik ELISA Kit, AI132708 ELISA Kit, AI463323 ELISA Kit, ENSMUSG00000074598 ELISA Kit, Gm10726 ELISA Kit, RGD1304890 ELISA Kit, UBFM1 ELISA Kit, ubfm1 ELISA Kit, wu:fa10f05 ELISA Kit, wu:fb72g01 ELISA Kit, zgc:55335 ELISA Kit, ubiquitin fold modifier 1 ELISA Kit, ubiquitin-fold modifier 1 ELISA Kit, ubiquitin-fold modifier ELISA Kit, ubiquitin-fold modifier 1 L homeolog ELISA Kit, UFM1 ELISA Kit, ufm1 ELISA Kit, CCP2 ELISA Kit, Ufm1 ELISA Kit, ufm1.L ELISA Kit, PR46a ELISA Kit

Gen-ID: 51569

UniProt: P61960