SLC32A1 ELISA Kit
SLC32A1
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN5690971
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Target
Alle SLC32A1 ELISA Kits anzeigen
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SLC32A1
(Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1))
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Reaktivität
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Human
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Nachweismethode
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Colorimetric
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Methodentyp
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Sandwich ELISA
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Applikation
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ELISA
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Verwendungszweck
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Human VIAAT/SLC32A1 ELISA Kit.
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Proben
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytische Methode
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Quantitative
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Spezifität
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This ELISA antibody pair recognizes Human VIAAT.
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Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Plattentyp
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Pre-coated
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Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Lagerung
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4 °C
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Haltbarkeit
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6 months
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Target
Alle SLC32A1 ELISA Kits anzeigen
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SLC32A1
(Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1))
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Andere Bezeichnung
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VIAAT (SLC32A1 (SLC32A1 Produkte)
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Synonyme
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VGAT ELISA Kit, VIAAT ELISA Kit, vGAT ELISA Kit, Ci-vGAT ELISA Kit, slc32a1 ELISA Kit, viaat ELISA Kit, MGC68938 ELISA Kit, vgat ELISA Kit, xVIAAT ELISA Kit, R75019 ELISA Kit, Viaat ELISA Kit, CG8394 ELISA Kit, CG8394.2 ELISA Kit, Dmel\\CG8394 ELISA Kit, Vgat ELISA Kit, zgc:158324 ELISA Kit, solute carrier family 32 member 1 ELISA Kit, vesicular GABA transporter ELISA Kit, solute carrier family 32 member 1 S homeolog ELISA Kit, vesicular inhibitory amino acid transporter ELISA Kit, Vesicular inhibitory amino acid transporter ELISA Kit, solute carrier family 32 (GABA vesicular transporter), member 1 ELISA Kit, Vesicular GABA Transporter ELISA Kit, SLC32A1 ELISA Kit, vgat ELISA Kit, Bm1_20950 ELISA Kit, slc32a1.S ELISA Kit, slc32a1 ELISA Kit, CpipJ_CPIJ002704 ELISA Kit, viaat ELISA Kit, Slc32a1 ELISA Kit, VGAT ELISA Kit, Tsp_04599 ELISA Kit
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Gen-ID
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140679
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UniProt
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Q9H598
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Pathways
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Proton Transport
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