DKK4 ELISA Kit

Produktnummer ABIN5692231

Produktdetails DKK4 ELISA Kit

Target: DKK4 (Dickkopf Homolog 4 (Xenopus Laevis) (DKK4))

Bindungsspezifität: AA 19-224

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Untere Nachweisgrenze: 156 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human DKK-4. 96wells/kit, with removable strips.

Marke: PicoKine™

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO, Immunogen sequence: L19-L224

Sensitivität: < 10 pg/mL

Bestandteile: 96-well plate precoated with antibody
lyophilized recombinant standard
biotinylated antibody (dilution 1:100)
Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
Sample diluent buffer
Antibody diluent buffer
ABC diluent buffer
TMB color developing agent
TMB stop solution
Adhesive cover

Anwendungsinformationen

Testdauer: 15 min

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Haltbarkeit: 12 months

Details zu DKK4

Target: DKK4 (Dickkopf Homolog 4 (Xenopus Laevis) (DKK4))

Andere Bezeichnung: DKK4 (DKK4 Produkte)

Synonyme: DKK-4 ELISA Kit, Dkk-4 ELISA Kit, RGD1563172 ELISA Kit, dickkopf WNT signaling pathway inhibitor 4 ELISA Kit, DKK4 ELISA Kit, Dkk4 ELISA Kit

Hintergrund:

Synonyms: Dickkopf-related protein 4, Dickkopf-4, Dkk-4, hDkk-4, Dickkopf-related protein 4 short form, DKK4

Tissue Specificity: Expressed in cerebellum, T-cells, esophagus and lung.

Background: DKK4 is a membersof the Dickkopf family which are negative regulators of the growth-promoting Wnt / beta-catenin signaling pathway. By FISH, the DKK4 gene was mapped to chromosome 8p11.2-p11.1. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. Activity of this protein is modulated by binding to the Wnt co-receptor and the co-factor kremen 2. The expression of DKK4 was significantly reduced in hepatocellular carcinomas (HCCs), particularly in HCCs that showed cytoplasmic beta- catenin accumulation. DKK4 expression was also reduced in HCC cell lines. Ectopic expression of DKK4 in 2 HCC cell lines reduced beta-catenin and cyclin D1 protein levels and attenuated beta-catenin activity in reporter gene assays.

Cellular Localisation: Secreted.

UniProt: Q9UBT3

Pathways: WNT Signalweg